Best Diethyl Pyrocarbonate CAS 1609-47-8

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Diethyl Pyrocarbonate
1609-47-8

Name: Diethyl Pyrocarbonate

Cas: 1609-47-8

Purity: 99%min

MOQ: 1KG

Directory Guidance on Diethyl Pyrocarbonate

Chemical Structure

Diethyl Pyrocarbonate

Basic Info:

CAS No:1609-47-8
Formula:C6H10O5
EINECS:216-542-8
Stock AvailabilityIn stock
Brand NameLook chem
OriginChina

Product Introduction:

Diethyl Pyrocarbonate (DEPC) is a colorless viscous liquid, also known as diethyl dicarbonate.

It is a strong but incomplete RNase inhibitor. It is an organic ester compound with a molecular formula of C6H10O5.

It is soluble in ethanol, ether, acetone and hydrocarbons, and slowly dissolves in water to undergo hydrolysis to generate ethanol and carbon dioxide.

DEPC can react with amines and sulfhydryl groups, so reagents containing Tris and DTT cannot be treated with DEPC.

Diethyl Pyrocarbonate is irritating and has a strong irritation to the eyes and airway mucosa. During the operation, it should be carried out under ventilated conditions as much as possible.

Nature and Specifications:

Product Packaging

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Product Usage:

  • Diethyl Pyrocarbonate is commonly made use of in the laboratory to suspend the RNase enzyme in water and lab equipment. It denatures the healthy protein by binding to the imidazole ring of histidine, the active group of the RNase, therefore inhibiting the activity of the enzyme.
  • DEPC (treated) water can be utilized for the dissolution of RNA precipitation, different response systems having RNA such as reverse transcription, annealing of siRNA, etc, as well as various other reaction systems that call for no RNase, proteinase and dnase.
  • Diethyl Pyrocarbonate can be utilized as a modification reagent for His and also Tyr deposits in proteins.
  • A long lasting probe utilized for architectural cleavage in dsDNA that partially or fully reacts with non-stacking bases.
  • Made use of as anti-bacterial ingredients, ribonuclease inhibitors, histidine deposit modifiers, and also reagents for transforming imines into carbamates.
  • DEPC derivatization of histidine is additionally used to examine the importance of histidine deposits in enzymes.
  • DEPC alteration of histidine can be reversed by therapy with 0.5 M hydroxylamine at neutral pH.
  • Diethyl Pyrocarbonate can also be used to probe the structure of double-stranded DNA.

Related References:

  1. Barry Wolf, Judith A. Lesnaw, Manfred E. Reichmann: Mechanism of Diethyl Pyrocarbonate inactivating bovine pancreatic ribonuclease-European Journal of Biochemistry.
  2. Diethyl Pyrocarbonate(DEPC)-Frequently Asked Questions Guide | sigma aldrich.
  3. Wikipedia

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