Deoxyribonuclease I [Dnase I] Powder CAS 9003-98-9 Supplier

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Deoxyribonuclease I
9003-98-9

Products Description

Name: Deoxyribonuclease I

Cas: 9003-98-9

Purity: 99%min

MOQ: 1KG

Basic Info:

CAS No:

9003-98-9

Formula:

N/A

EINECS:

232-667-0

Stock Availability

In stock

Brand Name

Look chem

Origin

China

 

Product Introduction:

Deoxyribonuclease I (DNase I) is an enzyme that cleaves DNA, an endonuclease found in a variety of cells and tissues, which cleaves the phosphodiester bond adjacent to the pyrimidine to produce a phosphate group at the 5’end Polynucleotides with hydroxyl groups at the 3’end, and the smallest average digestion product is polytetranucleotides.

DNase can catalyze various forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is affected by histones). The best working range is pH7-8. The activity of DNase depends on Ca2+ and can be activated by divalent metal ions such as Co 2+, Mn2+, Zn2+, etc.

5 mM Ca2+ can protect enzymes from being hydrolyzed. In the presence of Mn2+, two strands of DNA can be recognized at the same time and cut at almost the same site. Deoxyribonuclease I was first isolated from the pancreas, and the mammalian pancreas is also one of the most important sources of this enzyme.

Deoxyribonuclease I comes from bovine pancreas and is composed of four chromatographically distinguished components A, B, C, and D, with a molar ratio of 4:1:1, while the D component is very small. In molecular biology experiments, it is often used to remove DNA from proteins, or to introduce gaps in DNA to insert labeled bases into DNA.

Deoxyribonuclease I is supplied as a freeze-dried powder containing calcium chloride, with enzyme activity ≥2000 Kunitz Units/mg protein.

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Product Nature:

  • Density: 100g/mLat20°C
  • Storage conditions: -20°C
  • Solubility: Solublein0.15Msodiumchloride(5.0mg/L)clear.
  • Color: white
  • Merck: 13,2923

Specification:

ItemSpecification
Product NameDeoxyribonuclease I Powder
CAS No.9003-98-9
Appearance White crystalline powder
Content99% min
Shelf Life2 years
Packing1kg/bag,25kg/bag or as your requirements

Product function:

  • Deoxyribonuclease I can be used in biochemical research. It is often used clinically as an expectorant. It can also be used locally for abscesses, hematomas, and intrathecal injection to treat meningitis.
  • Studies have shown that deoxyribonuclease I powder from bovine pancreas has been used to study the two-dimensional zymogram analysis of Bacillus subtilis nuclease.
  • Deoxyribonuclease I from bovine pancreas has also been used to study the effects of minor groove and major groove binding drugs and intercalators on the DNA binding of the minor groove binding protein RecA and deoxyribonuclease I.
  • It acts on phosphodiester bonds, especially for bonds adjacent to pyrimidine nucleosides, to produce polynucleotides with free hydroxyl groups at the 3’end and phosphates at the 5’end.
  • DNase can be activated by divalent metals and inhibited by chelating compounds such as EDTA and sodium lauryl sulfate. Calcium ion at a concentration of 5mM can be used as a stabilizer to protect DNase from being decomposed by hydrolase.

About Dnase I Application Supplement:

  • Deoxyribonuclease I can be used to prepare RNA samples without DNA.
  • Dnase I can be used to remove possible DNA contamination such as genomic DNA from RNA samples before RT-PCR reaction.
  • Dnase I can be used to remove the DNA template after RNA transcription catalyzed by RNA Polymerases such as T7, T3, SP6, etc. in vitro.
  • DNase I’s footprint method studies DNA-protein interactions.
  • Notch translation method for labeling probes.
  • Generate a library of random fragments of DNA.
  • Partial shearing of genomic DNA was used as a positive control in the TUNEL test of apoptosis.

References:

  1. “Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
  2. GRCm38: Ensembl release 89: ENSMUSG00000005980– Ensembl, May 2017
  3. Wikipedia
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Service:

Pre-Sales Service

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* The sample supports testing and inspection.

* Each batch of products will be tested to ensure that its quality meets user needs.

*Packaging can also be made according to customer requirements.

*Any inquiries will be answered by our relevant personnel within 24 hours.

*We will provide you with commercial invoice, packing list, packing list, COA, health certificate and certificate of origin if you need it. If your market has other special requirements, please let us know.

 

After-Sales Service

*We will monitor the logistics information in real time and will share the information with you.

* You can consult us at any time if you have any questions about the product, and we will answer you in time.

*If you have any questions about the product, you can report it to us, we will deal with it in time for you, and the product can be returned.

* After the product is received, if you have any product quality problems, please contact us, we will deal with it for you as soon as possible.

Shipping:

1. ≤50kg, we recommend express delivery, usually called DDU service;

2. ≤500kg, air transportation is generally recommended, usually called FOB, CFR or CIF service;

3.>500kg, sea freight is generally recommended, usually called FOB, CFR or CIF service;

4. For high-value products, please choose air and express to ensure the safety of the products.

FAQ

Do you accept sample order?

We will make samples before mass production. After the samples are approved by the department and users, we will start mass production. During the production process, we will carry out 100% inspection, and then carry out random inspection before packaging.

How to confirm whether the product quality meets our needs before placing an order?

You can consult us to get free samples of some products, you only need to pay the freight or arrange express delivery to us and get the samples. You can send us the product specifications and requirements you need, and we will manufacture products according to your requirements.

What’s your MOQ?

Our MOQ is 1kg. But usually we can also accept a smaller quantity, such as 100g, if you need it, provided that the sample fee is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

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Shandong Look Chemical

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