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Luminol (Luminol), also known as luminol, chemical name is 3-Aminophthalhydrazide, chemical formula is C8H7N3O2.
3-Aminophthalhydrazide is a light yellow powder at area temperature level, which is a relatively stable synthetic natural substance.
3-Aminophthalhydrazide is a chemical fluorescent molecule, which can be converted into excited state aminophthalic acid in the presence of hydrogen peroxide molecule, which emits strong fluorescence.
Hydrogen peroxide is the product of many biooxidative reactions, so it is easy to link these biooxidative reactions with photodetection by introducing luminol.
For the blood that can not be observed with the naked eye at the crime scene, the luminol reagent can show an extremely percentage of blood (occult blood response).
At the same time, luminol is a solid weak acid, which has a specific bothersome effect on the eyes, skin and breathing system.
Luminol is extensively utilized in criminal investigation, bioengineering, chemical tracer as well as other fields.
Luminol was manufactured in 1853.
In 1928, chemists first discovered that this compound has the curious property that it produces blue light when it is oxidized.
A few years later on, a person thought of using this feature to discover bloodstains.
The luminol reagent we usually state is a combination of luminol as well as hydrogen peroxide (the major part of hydrogen peroxide), which is mostly used for blood testing in modern criminal examinations.
When luminol responds with hydroxide, a double negative ion (Dianion) is formed, which can be oxidized by the oxygen broken down by hydrogen peroxide, as well as the item is a natural peroxide.
The peroxide (assumed to be a cyclic inner peroxide) is extremely unsteady and decays quickly to nitrogen (oxidation of luminol by an organic oxidant such as dimethyl sulfoxide does not create nitrogen, however nitrogen-containing organics).
The ecstatic state of 3-aminophthalic acid is generated (the triplet divalent anion (T1) system cross-interacts to create a singlet divalent anion (S1)).
In the change from the thrilled state to the ground state, the released energy exists in the form of photons with a wavelength in the blue part of visible light.
Luminol will only radiance if it has been treated with an oxidizing representative.
A mixed liquid service of hydrogen peroxide and also a hydroxide base is typically utilized as the activator.
Under the catalysis of iron compounds, hydrogen peroxide is decayed into oxygen and water: 2H2O2 → O2 ↑ +2 water Busy, potassium ferricyanide is often used as the resource of catalyst iron, while the driver in forensic medication takes place to be iron in hemoglobin.
Enzymes in several organic systems also militarize the decay of hydrogen peroxide.
The luminol reagent utilizes a reagent that recognizes blood. Even if the blood is wiped, the heme in the blood will certainly stay. When the luminol reagent is splashed on the heme, it will oxidize with energetic oxygen as well as launch blue-violet fluorescence.
Called the luminol response, 3-Aminophthalhydrazide is an organic compound utilized to identify blood.
The examination is very delicate, able to spot blood as low as one component per million, as well as even a small drop of blood can be detected in a huge container of water.
Luminol is utilized as one of the most commonly made use of chemiluminescence reagent due to the fact that it has the qualities of being oxidized by other materials to generate light, and also has many advantages such as high luminescence quantum return, simple synthesis, and also good water solubility.
A lot of luminol chemiluminescence systems are often made use of in the determination of biochemical experiments.
Additionally, they can likewise be utilized as detectors for some splitting up strategies such as capillary electrophoresis and high performance fluid chromatography.
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